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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: J Cell Physiol. 2020 Apr 2;235(11):8334–8344. doi: 10.1002/jcp.29677

Figure 2.

Figure 2.

Metformin promotes the intracellular redistribution of E-cadherin, β-catenin and p120-catenin and E-cadherin Ser838/840 phosphorylation. (A) SW-480 cells incubated for 16 with 2.0 mM metformin underwent subcellular fractionation and the content of E-cadherin, β-catenin, p120-catenin and E-cadherin phosphorylated at Ser838/840 determined in the cytosolic and membrane fractions of three independent experiments by Western blot. Bars represent the mean ± SE as percentage of the total (Cyt. + memb.) of each examined protein, with or without metformin treatment. p(Bonferroni) ****<0.0001, ***< 0.001, **<0.01. α-Tubulin and the plasma membrane Ca2+ ATPase (PMCA) were used as cytosolic and membrane markers and as controls for loading normalization. (B) Total SW-480 cells lysates incubated for 16 with 2.0 mM metformin were analyzed by Western blot using rabbit antibodies against E-cadherin phospho-Ser838/840 (pE-Cadherin Ser838/840) and a murine monoclonal antibody against E-cadherin. Bars represent the mean ± SE relative cellular content of three independent experiments normalized by α-tubulin. p (Bonferroni) **< 0.01. RIS= Relative Intensity Signal.