Skip to main content
. Author manuscript; available in PMC: 2021 Jan 8.
Published in final edited form as: Leukemia. 2020 Apr 1;35(1):177–188. doi: 10.1038/s41375-020-0809-x

Figure 3. CRBN expression does not regulate phosphorylation or protein expression of Zap-70.

Figure 3.

(a) Jurkat cells were transfected with scrambled siRNA (Scsi) or CRBN siRNA (CRBNsi). Whole cell lysates were subjected to immunoblotting using indicated Abs. (b) Jurkat cells transfected with Scsi or CRBNsi were cultured with Pom (1 μM) for 16h. Whole cell lysates were subjected to immunoblotting using indicated Abs. (c) Jurkat cells were transfected with Scsi or Zap-70 siRNA (Zap-70si) (left panel). The transfectants were further cultured for 72h, and cell growth was assessed by MTT assay (right panel). Data are representative of three independent experiments and values are expressed in mean ± SD.