Fig. 2.

2’-FL and 6’-SL supplementations in nutritional formula prevents small intestinal mucosal injury in mice induced to develop necrotizing enterocolitis (NEC). ai-v, i’-v’ immunofluorescence images of 3’-Nitrotryosine (3’-NT, green fluorescence) as an indicator of oxidative injury, bi-v, i’-v’ immunofluorescence images of TUNEL (green fluorescence) as an indicator of apoptosis, in ileal sections of neonatal mice subjected to no treatment (Ctrl, control–breast-fed) or experimental NEC without or with supplementation with 2’-FL (10mg/ml/), 6’-SL (10mg/ml), or 2’-FL+6’-SL (5mg/ml, each), c quantification of fluorescent intensity of 3’-NT staining, d quantification of fluorescent intensity of TUNEL staining, measured using ImageJ software, *** P<0·001. Each dot in dot-graphs represents data from an individual mouse.