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. Author manuscript; available in PMC: 2021 Feb 14.
Published in final edited form as: Pediatr Res. 2020 Mar 27:10.1038/s41390-020-0852-3. doi: 10.1038/s41390-020-0852-3

Fig. 4.

Fig. 4

2’-FL and 6’-SL but not lactose prevents LPS induced NF-kB translocation in cultured intestinal epithelial (IEC6) cells. ai-vi, ai’-vi’ photomicrographs of IEC6 cells immunostained for NF-kB p65, b quantification of fluorescence intensity measured using ImageJ, *** P<0·001. Each dot in dot-graphs represents data from an individual cell. C–d: 2’-FL and 6’-SL but not lactose prevents LPS induced apoptosis in cultured intestinal epithelial (IEC6) cells. ci-vi, i’-vi’ photomicrographs of IEC6 cells immuno-stained with in Situ death staining kit, TUNEL, d quantification of fluorescent intensity measured using ImageJ software, *** P<0·001. Each dot represents data from an individual cell.