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. 2020 Oct 1;3:547. doi: 10.1038/s42003-020-01266-9

Fig. 2. Tripodal peptidyl compounds activate the DegP protease.

Fig. 2

Data are presented as dot plots with mean ±1 SD (n = 3 independent experiments). a, b Activation effects of compounds were monitored by measuring cleavage rates of the reporter peptide (100 μM) by DegP (1 μM for TMB_CKYRKL and TMB_CYRKL (a); 10 μM for Lpp+Leu, TMB_CRKL, and TMB_CKL (b)) with increasing concentrations of Lpp+Leu or tripodal compounds. The insets show close-up views. c Effect of alanine substitution on each position of the pentapeptide (CYRKL) for DegP activation. d DegP activation effects of TMB_CYYKI or its variants with an alanine substitution were monitored as described in Fig. 2a. e Degradation of denatured OmpA (2 μM) by DegP (5 or 0 μM) was monitored by SDS-PAGE in the absence or presence of TMB_CYYKI (10 μM).