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. 2020 Oct 1;3:547. doi: 10.1038/s42003-020-01266-9

Fig. 6. The compound with three arms has the best activity and properties different from those with one or two arms.

Fig. 6

a Structures of compounds with three, two, or one peptidyl arm(s) (TMB_CYYKI, DMB_CYYKI, or MMB_CYYKI, respectively). b Activation effects of TMB_CYYKI, DMB_CYYKI, and MMB_CYYKI were monitored by measuring cleavage rates of the reporter peptide (100 µM) by DegP (1 µM) with increasing concentrations of compounds. Data are presented as dot plots with mean ± 1 SD (n = 3 independent experiments). c–g Isothermal titration calorimetry (ITC) experiments of TMB_CYYKI (c), 18–58 (d), DMB_CYYKI (e, g for 0–2 and 0–0.4 molar ratio, respectively), and MMB_CYYKI (f) with DegPS210A. Data were fitted to the single binding site model. h DegP assembly was monitored by measuring fluorescence resonance energy transfer (FRET) of the donor- and acceptor-labeled DegP variants (0.1 M total) with increasing concentrations of 18–58, TMB_CYYKI, DMB_CYYKI, or MMB_CYYKI. Data were fitted to the Hill equation. Data are presented as dot plots with mean ± 1 SD (n = 3 independent experiments).