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. 2020 Oct 1;10:16316. doi: 10.1038/s41598-020-73330-7

Figure 3.

Figure 3

PGRMC1 regulates PEPCK via adenylate cyclase. (A) Western blot analysis and quantification of PGRMC1 and PEPCK in WT versus WT + AG-205 hepatocyte. AG-205 (10 µg/ml) was treated in DMEM-low glucose medium for 18 h. β-actin was used as an internal control. Values represent means ± SD. *p < 0.05 versus WT hepatocyte. (B) Western blot analysis and quantification of PGRMC1 and PEPCK in WT versus Pgrmc1 KO versus Pgrmc1 KO + AG-205 hepatocyte. AG-205 (10 µg/ml) was treated in DMEM-low glucose medium for 18 h. β-actin was used as an internal control. Values represent means ± SD. *p < 0.05 versus WT hepatocyte. (C) Western blot analysis and quantification of PGRMC1 and PEPCK in WT, Pgrmc1 KO, WT + MDL (20 µM), and Pgrmc1 KO + MDL (20 µM) groups. β-actin was used as an internal control. Values represent means ± SD. *p < 0.05 versus WT hepatocyte. All experiments were repeated at least 3 times.