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. 2020 Oct 1;10:16316. doi: 10.1038/s41598-020-73330-7

Figure 4.

Figure 4

PGRMC1 regulates PEPCK via phosphorylation of CREB. (A) Western blot analysis and quantification of PGRMC1, phosphorylated/total CREB, and PEPCK in Control siRNA (Con) versus PGRMC1 siRNA transfection group. After PGRMC1 knockdown, cells were incubated in DMEM-low glucose medium (50 mg/dl) for 18 h. β-actin was used as an internal control. Values represent means ± SD. *p < 0.05 versus Con. (B) Western blot analysis and quantification of PGRMC1, phosphorylated/total CREB, and PEPCK in Control (Con) versus PGRMC1 overexpression group. After plasmid transfection, cells were incubated in DMEM-low glucose medium for 18 h. β-actin was used as an internal control. Values represent means ± SD. *p < 0.05 versus Con. (C) Western blot analysis and quantification of PGRMC1, phosphorylated/total CREB, and PEPCK in WT versus Pgrmc1 KO hepatocytes. Cells were incubated in DMEM-low glucose medium for 18 h. β-actin was used as an internal control. Values represent means ± SD. *p < 0.05 versus WT hepatocyte. All experiments were repeated at least 3 times.