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. 2020 Oct 1;10:16293. doi: 10.1038/s41598-020-73207-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Drift time profile for the 8+ charge state of α-syn with different numbers of Cu²⁺ bound. The control shows the conformations of α-syn without metal ions present in the sample. (b) Drift times are converted to CCS plots after calibration. ‘0’ indicates the presence of CuCl₂ but shows the unbound state. Different conformations are labelled “A” (most extended) to “D” (most compact). Intensity scale as in Fig. 1b.