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. 2020 Oct 1;10:16293. doi: 10.1038/s41598-020-73207-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Mass spectrum of the 8+ charge state of α syn without ions bound, in absence (control) and presence of 5 mM (NH₄)₂Fe(SO₄)₂. Binding of Fe²⁺ is indicated in grey while SO₄²⁻ binding is indicated in blue. Black dashed lines indicate a peak with one or two SO₄²⁻ bound and one Fe²⁺ bound. (b) Comparison of drift time plots of the 8+ charge state of α syn in the absence (Control) and presence of 5 mM (NH₄)₂Fe(SO₄)₂ resulting in various bound states. Different conformations are labelled “A” (most extended) to “D” or “D¹/D²” respectively (most compact).