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. 2020 Oct 1;10:16371. doi: 10.1038/s41598-020-73370-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Uracil incision activities and UDG protein levels in NMIBC and their corresponding normal tissues. (a) Representative gel for uracil incision. Incision products are 25mer derived from cleaved ³²P-labeled 51mer DNA substrate containing uracil at position 26. N, the corresponding normal tissue. Quantitation of uracil incision from high-grade (b), low-grade (c) and Ta/T1 stages (d) of all NMIBC and their corresponding normal tissues (fmol/μg/min). (e) Western blot analysis of UDG and β-actin in NMIBC and their corresponding normal tissues. Low-grade sample numbers are 2, 4, 9–12; high-grade sample numbers are 1, 3, 5–8, 13–17. (f) Representative gel for single-stranded uracil incision. (g) Quantitation of single-stranded uracil incision. The full-length gels/blots are presented in Supplementary Fig. 2. Radioactive gels were visualized by Typhoon FLA 9500 PhosphorImager and analyzed using the ImageQuant software TL v8.1 (https://www.cytivalifesciences.com/en/us/shop/protein-analysis/molecular-imaging-for-proteins/imaging-software/imagequant-tl-8-1-p-00110).