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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: J Cell Physiol. 2020 Apr 24;235(11):8520–8532. doi: 10.1002/jcp.29696

Figure 1.

Figure 1.

Weight and documentation of DNA recombination of Nfatc1 and Nfatc2 alleles by BGLAP-driven Cre. (A) Body weight of BGLAP-Cre;Nfatc1Δ/Δ, BGLAP-Cre;Nfatc2Δ/Δ and BGLAP-Cre;Nfatc1Δ/Δ;Nfatc2Δ/Δ and sex-matched control Nfatc1loxP/loxP, Nfatc2loxP/loxP and Nfatc1loxP/loxP;Nfatc2loxP/loxP littermates. Values are means ± SD; n = 3 control and n = 6 Nfatc1Δ/Δ; n = 5 control and n = 7 Nfatc2Δ/Δ and n = 3 control and n= 9 Nfatc1Δ/Δ;Nfatc2Δ/Δ. (B) DNA from tibiae were obtained from BGLAP-Cre;Nfatc1Δ/Δ and DNA from calvariae were obtained from BGLAP-Cre;Nfatc2Δ/Δ and from BGLAP-Cre;Nfatc1Δ/Δ;Nfatc2Δ/Δ neonates and from their respective (non-Cre) Nfatc1loxP/loxP, Nfatc2loxP/loxP and Nfatc1loxP/loxP;Nfatc2loxP/loxP controls. Cre-dependent DNA recombination was demonstrated by gel electrophoresis of PCR amplification products obtained with primers for the unrecombined Nfatc1loxP and Nfatc2loxP and for the Cre-mediated recombined Nfatc1Δ and Nfatc2Δ alleles. In Figure 1B and 1F left, the arrow heads indicate the position of the 410 base pair (bp) amplicon verifying the unrecombined Nfatc1loxP allele and 510 bp amplicon verifying the Cre-mediated recombined Nfatc1Δ allele. In Figure 1D and right, the arrow heads indicate the 2.3 kilobase (kb) amplicon verifying the unrecombined Nfatc2loxP allele, and the 0.36 kb amplicon verifying the Cre-mediated recombined Nfatc2Δ allele.