Figure 5.

Bone marrow stromal cells harvested from femurs of 6 to 8 week old male Nfatc1^loxP/loxP;Nfatc2^loxP/loxP mice were cultured to subconfluence, transduced with Ad-CMV-Cre (Nfatc1^Δ/Δ;Nfatc2^Δ/Δ, black bars) or Ad-CMV-GFP (control, white bars) and cultured under conditions favoring osteoblastogenesis following confluence (Day 0). In Panel A, total RNA was extracted and gene expression determined by qRT-PCR. Data are expressed as Nfatc1, Nfatc2, Alpl and Bglap copy number corrected for Rpl38 copy number. Values are means ± SD; n = 4. *Significantly different between Nfatc1^Δ/Δ;Nfatc2^Δ/Δ and control, p < 0.05. In Panel B, a representative image shows control and Nfatc1^Δ/Δ;Nfatc2^Δ/Δ 14 day cultures stained with alizarin red.