Table 1.

Primers used for allele identification and for qRT-PCR.

A. rimers for allele identification and DNA recombination
Allele	Strand	Sequence	Amplicon Size (bp)
BGLAP-Cre transgene	Forward Reverse	5’-CAAATAGCCCTGCAGAT-3’ 5’-TGATACAAGGGACATCTTCC-3’	300
Nfatc1loxP	Forward Reverse	5’-GGACAGTCTAAGGCCTGCTG-3’ 5’-ACCCCACATCCCAGAGTGA-3’	Nfatc1WT = 215 Nfatc1loxP = 300
Nfatc2loxP	Forward Reverse	5’-GGTGTCTCCCTCAAATGTTCA-3’ 5’-GCGAATATGAGGACCCAAGT-3’	Nfatc2WT = 160 Nfatc2loxP= 250
LoxP recombination of Nfatc1loxP	Forward Forward Reverse	5’-AAGGAATTACTGGGAAGCCTGGCA-3’ 5’-AGGGACTATCATTTGGCAGGGACA-3’ 5’-ACAGGAAACAGCTCTGTTCCACAC-3’	Recombined = 510 (Nfatc1Δ) Not recombined = 410 (Nfatc1loxP)
LoxP recombination of Nfatc2loxP	Forward Reverse	5’-AGCACAATGCCCATGTTCAC-3’ 5’-GGTGTCTCCCTCAAATGTTCA-3’	Recombined = 358 (Nfatc2Δ) Not recombined = 2342 (Nfatc2loxP)

B. Primers for qRT-PCR and GenBank accession numbers for the transcript variants recognized by primer pairs
Gene	Strand	Primer Sequence	GenBank Accession Number
Alp1	Forward Reverse	5’-CGGTTAGGGCGTCTCCACAGTAAC[FAM]G-3’ 5’-CTTGGAGAGGGCCACAAAGG-3’	NM_007431
Bglap	Forward Reverse	5’-GACTCCGGCGCTACCTTGGGTAAG-3’ 5’-CCCAGCACAACTCCTCCCTA-3’	NM_001037939
Nfatc1	Forward Reverse	5’-GCGCAAGTACAGTCTCAATGGCC-3’ 5’-GGATGGTGTGGGTGAGTGGT-3’	NM_198429; NM_001164110; NM_001164111; NM_001164112; NM_00116641091; NM_016791
Nfatc2	Forward Reverse	5’-AGAACAACATGAGAGCCACCATC-3’ 5’-AGCTCGATGTCAGCGTTTCG-3’	NM_010899
Rpl38	Forward Reverse	5’-AGAACAAGGATAATGTGAAGTTCAAGGTTC-3’ 5’-CTGCTTCAGCTTCTCTGCCTTT-3’	NM_001048057; NM_001048058; NM_023372