Table 2.
Lumbar 3 (L3) assessed by μCT of BGLAP-Cre;Nfatc1Δ/Δ and BGLAP-Cre;Nfatc2Δ/Δ male and female mice and sex-matched Nfatc1loxP/loxP or Nfatc2loxP/loxP littermate controls.
| Nfatc1 | Males | Females | ||
|---|---|---|---|---|
| Control n = 8 |
Nfatc1Δ/Δ n = 8 |
Control n = 9 |
Nfatc1Δ/Δ n = 6 |
|
| Distal Femur Trabecular Bone | ||||
| Bone Volume/Total Volume (%) | 12.6 ± 0.9 | 10.8 ± 1.4* | 13.0 ± 2.0 | 10.9 ± 1.0* |
| Trabecular Separation (μm) | 185 ± 9 | 187 ± 10 | 220 ± 10 | 239 ± 14* |
| Trabecular Number (1/mm) | 5.2 ± 0.3 | 5.2 ± 0.3 | 4.5 ± 0.2 | 4.1 ± 0.2* |
| Trabecular Thickness (μm) | 28 ± 1 | 26 ± 1* | 31 ± 3 | 31 ± 1 |
| Connectivity Density (1/mm3) | 418 ± 48 | 422 ± 52 | 380 ± 72 | 306 ± 63 |
| Structure Model Index | 1.2 ± 0.1 | 1.4 ± 0.1* | 1.2 ± 0.3 | 1.3 ± 0.1 |
| Density of Material (mg HA/cm3) | 947 ± 11 | 960 ± 21 | 928 ± 33 | 938 ± 10 |
| Nfatc2 | Males | Females | ||
|---|---|---|---|---|
| Control n = 8 |
Nfatc2Δ/Δ n = 9 |
Control n = 8 |
Nfatc2Δ/Δ n = 8 |
|
| Distal Femur Trabecular Bone | ||||
| Bone Volume/Total Volume (%) | 11.3 ± 0.8 | 10.2 ± 1.3+ | 8.3 ± 0.7 | 6.9 ± 1.2* |
| Trabecular Separation (μm) | 201 ± 9 | 199 ± 13 | 255 ± 16 | 257 ± 15 |
| Trabecular Number (1/mm) | 5.0 ± 0.2 | 5.0 ± 0.3 | 4.0 ± 0.3 | 3.9 ± 0.2 |
| Trabecular Thickness (μm) | 29 ± 1 | 28 ± 2 | 31 ± 2 | 28 ± 1* |
| Connectivity Density (1/mm3) | 350 ± 44 | 342 ± 86 | 237 ± 44 | 252 ± 70 |
| Structure Model Index | 1.3 ± 0.2 | 1.5 ± 0.2 | 1.7 ± 0.1 | 1.9 ± 0.2* |
| Density of Material (mg HA/cm3) | 1012 ± 18 | 1009 ± 22 | 973 ± 24 | 977 ± 29 |
μCT was performed in L3 vertebral bodies from 3 month old male and female BGLAP-Cre;Nfatc1Δ/Δ mice and 4 month old BGLAP-Cre;Nfatc2Δ/Δ mice and sex-matched Nfatc1loxP/loxP or Nfatc2loxP/loxP littermates. Values are means ± SD.
*
Significantly different between control and BGLAP-Cre;Nfatc1Δ/Δ or control and BGLAP-Cre;Nfatc2Δ/Δ, p < 0.05 by unpaired t-test;
+
p = 0.058.