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. Author manuscript; available in PMC: 2021 Jan 13.
Published in final edited form as: Nat Microbiol. 2020 Jul 13;5(10):1217–1231. doi: 10.1038/s41564-020-0750-9

Extended Data Fig. 5. MS2 evidence for ubiquitination site localization in RALY (a) and hnRNP-C (b-f) peptides.

Extended Data Fig. 5

Spectra were obtained from LC-MS/MS analyses using collision-induced dissociation (CID) at 35%, and identified in MaxQuant 1.6.0.1. All modified residues can be confidently identified by confirming ions, except for hnRNP-C K198 (d), which lacks ions to distinguish between K197 and K198. Best evidence spectra were selected for annotation of b-ion (blue) and y-ion (red) series and their masses for singly- and doubly-charged fragments. All data are representative of three biologically independent experiments.