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. 2020 Mar 31;27(12):910–922. doi: 10.1038/s41417-020-0171-1

Fig. 1. Characterization of dual targeting MV-CD46-muPA.

Fig. 1

a MV-CD46-muPA was generated by displaying the amino terminal fragment (ATF) of murine uPA (flanked by the SfiI/NotI restriction sites) as a C-terminal extension of unmodified MV-H glycoprotein, as in methods. b In vitro tumor and species specificity of MV-GFP, MV-muPA, and MV-CD46-muPA. Human CD46 (hCD46) and mouse uPAR expression were detected by FACS. Human cancer cells (MDA-MB-231, 786-O, HT-29), human cancer associated fibroblast CAF23, murine cancer cells (4T1, CT-26), and murine fibroblast 3T3 cells were infected with MV-GFP, MV-muPA, or MV-CD46-muPA at an MOI = 1 and photographed 48 h after infection using a fluorescent microscope. Pictures are representative of independent experiments performed in triplicate. Scale bar = 100 μm.