Fig. 1.

MPL/TDCM-induced accumulation of activated small peritoneal macrophages and monocytes is not reduced in FcγR^−/− and TLR4^−/− mice. a–f Inflammatory monocytes (CD11b⁺ Ly6G⁻ CD11c⁻ B220⁻ Ly6C⁺ SSC^low) and macrophages (CD11b⁺ Ly6G⁻ CD11c⁻ B220⁻ Ly6C^low SSC^low) were enumerated in peritoneal cavities on day 1 post TA3-Ha cell challenge ± MPL/TDCM treatment. a, d Monocyte and macrophage numbers in WT, FcγR^−/− and TLR4^−/− mice. b, e Small peritoneal macrophages (SPM; CD11b^midF4/80^mid) and large peritoneal macrophages (LPM; CD11b^hiF4/80^hi) were further quantified. c, f MHC II⁺ SPM and monocyte cell numbers on day 1 post-tumor challenge. Data representative of two experiments. Values represent means ± SEM. Asterisks (*) in a–f indicate significant differences between indicated groups (p < 0.05; n = 3–5 mice/group)