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. 2020 Oct 1;10:16360. doi: 10.1038/s41598-020-73477-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Intrauterine exposure to DBP decreased UCP1 protein expression in offspring. (A) The relative mRNA expression levels of UCP1, Pgc-1α, Prdm16 and Cidea were analyzed by RT-PCR. (B) The protein levels of UCP1 was assessed by western blot. Their corresponding full-length blots are presented in Supplementary Fig. S1. (C) The relative mRNA expression levels of Bip and Chop were analyzed by RT-PCR. (D) The protein levels of Bip and Chop was analyzed by Western blot. Their corresponding full-length blots are presented in Supplementary Fig. S2. All data above were expressed as the mean ± SD of three independent experiments. Data are expressed as the mean ± SD, n = 10 per group, **p < 0.01 versus the Ctrl group.