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Primer sequences used for RT-PCR.
| Gene | Primer sequences (5′-3′) | Length |
|---|---|---|
| UCP1 | ||
| F | CACCATGGTGAACCCGACAACTTCC | 25 |
| R | TTATGTGGTACAATCCACTG | 20 |
| Pgc-1α | ||
| F | AACAATGAGCCTGCGAAC | 18 |
| R | CCTCGTTGTCAGTGGTCA | 18 |
| Prdm16 | ||
| F | CAGCACGGTGAAGCCATTC | 19 |
| R | GCGTGCATCCGCTTGTG | 17 |
| Cidea | ||
| F | AGGCCGTGTTAAGGAATCTG | 20 |
| R | CCCAGTACTCGGAGCATGTA | 20 |
| Bip | ||
| F | TGAAACTGTGGGAGGAGTCA | 20 |
| R | TTCAGCTGTCACTCGGAGAA | 20 |
| Chop | ||
| F | CTGCCTTTCACCTTGGAGAC | 20 |
| R | CGTTTCCTGGGGATGAGATA | 20 |
| β-actin | ||
| F | CATCCGTAAAGACCTCTATGCCAAC | 25 |
| R | ATGGAGCCACCGATCCACA | 19 |
