Figure 7.

Effect of U0126 ERK inhibitor on on-chip human dermal fibroblast cell migration and wound closure. (A) Representative phase contrast images of wound defects up to 20 h for dermal fibroblast monolayers treated with 10 µM and 20 µM U0126. The wound edges are highlighted with dashed lines. (Scale bar = 200 µm). (B) Comparison of wound closure of dermal fibroblast monolayers in the presence of 10 µM and 20 µM U0126 up to 20 h post-wounding. Data is expressed as mean ± SD. Data sets were tested with unpaired student’s t-test with 99% confidence level, ns non-significant, **p < 0.01, ***p < 0.001, ****p < 0.0001; (control n = 6; U0126 n = 4). (C) Comparison of cell number in defined wound areas of dermal fibroblast monolayers in the presence of 10 and 20 µM U0126 at 0 and 20 h. Data is expressed as mean ± SD. Data sets were tested with unpaired student’s t-test with 99% confidence level, ns = non-significant, **p < 0.01, ***p < 0.001, ****p < 0.0001; (control n = 4; U0126 (10 µM) n = 3; U0126 (20 µM) n = 4).