Extended Data Figure 2. HDAC activity and expression in IECs lack sensitivity to multiple bacterial stimuli.

(a) HDAC activity of mouse colonoid lysate treated with 1 μg/ml vehicle, butyrate, flagellin, LPS, or Pam3csk4. n=6/treatment. **p=0.0008. (b) Western blot and (c-e) qPCR analyses of HDAC1, HDAC2, and HDAC3 in colonoids following treatment with vehicle (n=9), butyrate (n=6), flagellin (n=6), LPS (n=6), or Pam3csk4 (n=6); all at 1 μg/ml for 5 hours. For gel source data, see Supplementary Fig. 1. (f, g) HDAC activity in IECs harvested from (f) floxed TLR4^FF and IEC-specific toll-like receptor 4 knockout (TLR4^ΔIEC) mice (n=3/genotype) or (g) wildtype and Myd88 knockout (Myd88^−/−) mice (n=4/genotype). All graphs are mean of biologic replicates ± s.e.m.; unpaired two-tailed t test. Data were independently repeated four (a) or two (b-g) times with similar results. **p ≤ 0.01.