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. Author manuscript; available in PMC: 2021 Jan 30.
Published in final edited form as: Nature. 2020 Jul 30;586(7827):108–112. doi: 10.1038/s41586-020-2604-2

Extended Data Figure 4. Inositol phosphate induces HDAC enzymatic activity without altering expression in IECs.

Extended Data Figure 4.

(a) HDAC activity in mouse colonoids following incubation with increasing doses of IP3 for 5 hours (Vehicle: n=8; 100nM IP3: n=5, *p=0.0062; 100μM IP3: n=6, *p=0.0147). (b) mRNA expression of HDAC1, HDAC2 and HDAC3 in mouse colonoids following incubation with IP3 for 5 hours at indicated dose (Vehicle: n=5; IP3: n=6/dose). (c) Western blot of HDAC1, HDAC2 and HDAC3 in colonoids from (a). For gel source data, see Supplementary Fig. 1. All graphs are mean of biologic replicates ± s.e.m.; unpaired two-tailed t test. Data were independently repeated three times with similar results. *p ≤ 0.05, **p ≤ 0.01.