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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Apoptosis. 2020 Oct;25(9-10):625–631. doi: 10.1007/s10495-020-01627-z

Figure 2. The role of BAX and BAK in the combined treatment of ERA and TRAIL-induced apoptosis.

Figure 2.

HCT116 WT, BAX−/− single knockout, or BAX−/−/BAK−/− double knockout cells (A) or HCT116 WT, BAK−/− or BAX−/−/BAK−/− cells (B) were pretreated with ERA (50 μM) for 20 h and then exposed to TRAIL (5 ng/ml) for an additional 4 h. Cell survival was analyzed using trypan blue exclusion assay (left panels). Whole-cell lysates were analyzed with immunoblotting assay using indicated antibodies (right panels). Error bars represent the mean ± SD from triplicate experiments. For statistical analysis, Student's t-test (two-sided, paired) was used. p-values: *P<0.05, **P < 0.01, ***P < 0.001.