Table 1.

Control and UPJO patient characteristics

	Control	UPJO
Number of samples	22	22
Sex	M	M
Age - Average (SD)	8.3 (3.0) months	6.0 (3.8) months
Weight - Average (SD)	8.5 (1.3) kg	7.3 (1.7) kg
Patients	Hypospadias repair	Confirmed obstruction - Mag-3 t1/2 >20min with differential function <40% in the affected side
Notes	Control-12 was used for mass spectrometric analysis only. Remaining samples were used for both mass spectrometric analysis and ELISA.	UPJO-6, 7, 8 and 21 were used for mass spectrometric analysis only, while UPJO-1 was used for ELISA only. Remaining samples were used for both the analyses.

Due to the limited availability, some of the samples were used for either mass spectrometric analysis or ELISA and have been identified in the notes.