Fig. 4.
Low concentration IL-1β increases IAPP release in vitro. (a–d) Treatment of hIAPP-expressing mouse islets with 4 pg/ml IL-1β (black squares) increased release of IAPP (a) and insulin (b) into the media over 48 h in culture when compared with vehicle treatment (black circles). At the end of the 48 h culture period, the islet content of IAPP (c) and insulin (d) protein did not differ between groups. (e, f) Iapp (e) and Ins2 (f) mRNA expression were decreased in IL-1β-treated (black squares) compared with vehicle-treated (black circles) islets (data normalised to 18S rRNA levels and expressed as fold relative to control using the 2−ΔΔCt method). Data are presented as mean ± SEM. n=4–9. *p<0.05, **p<0.01