Fig. 6.
Low concentration IL-1β does not alter the UPR or processing enzyme gene expression in hIAPP-expressing islets. (a–c) Treatment of hIAPP-expressing mouse islets with low concentration IL-1β for 48 h (black squares) significantly increased Nos2 mRNA expression, compared with vehicle treatment (black circles) (a), but mRNA expression of Atf4 (b) or Ddit3 (c), markers of the UPR, remained unaltered. (d–f) Similarly, mRNA expression of the proIAPP processing enzymes Pcsk1 (d), Pcsk2 (e) and Cpe (f) was unchanged in hIAPP-expressing islets following vehicle treatment (black circles) vs treatment with 4 pg/ml IL-1β (black squares). Data are normalised to 18S rRNA levels and expressed as fold relative to control using the 2−ΔΔCt method. Data are presented as mean ± SEM. n=5. **p<0.01