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. Author manuscript; available in PMC: 2021 Mar 2.
Published in final edited form as: Nature. 2020 Sep 2;585(7826):614–619. doi: 10.1038/s41586-020-2650-9

Extended Data Figure 8. Fluorescence microscopy of tobacco alkaloid transporters expressed in CSY1296 for alleviation of vacuolar TA transport limitations.

Extended Data Figure 8.

C-terminal GFP fusions of (a) NtJAT1 and (b) NtMATE2 were expressed from low-copy plasmids in CSY1296. Strains were cultured, imaged, and analyzed via widefield epifluorescence microscopy and 2D deconvolution analysis as described in Online Methods. Images are representative of two independent experiments. Scale bar, 5 μm.