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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Aug 13;40(10):2494–2507. doi: 10.1161/ATVBAHA.120.314955

Figure 4. BAF60a regulates cathepsin S expression in VSMCs.

Figure 4.

A, HASMCs were transfected with siControl or siBAF60a (30 nM). After 72 hours, CTSS mRNA expression was determined by qPCR. n=3. B, HASMCs were infected with AdGFP or AdBAF60a (30 MOI). After 48 hours, CTSS mRNA expression was determined by qPCR. n=3. C-D, HASMCs were transfected with siControl or siBAF60a (30 nM). After 48 hours, cells were treated with or without TNFα (20 ng/ml) for 24h. C, Cathepsin S protein level was determined by Western blot. n=3. D, Cathepsin S concentration in the culture medium was measured by ELISA. n=3. Data are presented as mean ± SEM. Student’s t-test for A-B, two-way-ANOVA followed by Holm-Sidak post hoc analysis for C-D.