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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Angiogenesis. 2020 Aug 11;23(4):685–698. doi: 10.1007/s10456-020-09740-y

Fig. 5. IQGAP1 regulates VEGF-mediated VEGFR2 activation and Rac1 activation.

Fig. 5.

(a–b) co-immunoprecipitation of VEGFR2 and IQGAP1 and phosphorylated VEGFR2 (p-VEGFR2) and western blots of total VEGFR2, IQGAP1, and β-actin in CECs transfected with IQGAP1 siRNA or control siRNA and treated with VEGF for 15 mins; (c–d) co-immunoprecipitation of GTP-Rac1 and IQGAP1, and GTP-Rac1 determined by immunoprecipitation with anti-GTP-Rac1 and western blot of total Rac1 in CECs transfected with IQGAP1 siRNA or control siRNA and treated with VEGF for 30 mins; (e) cell migration toward VEGF of CECs transfected with IQGAP1 siRNA or control siRNA and (f) tube formation induced by VEGF of CECs transduced with adenovirus-delivered IQGAP1shRNA or controlshRNA (**p<0.01, ***p<0.001vs. PBS of controlsiRNA, ††p< 0.01 vs. PBS of IQGAP1siRNA, ‡p<0.05, ‡‡‡p<0.001 vs. VEGF of ControlsiRNA; ##p<0.01 vs. PBS of ControlshRNA and $ $p<0.01 vs. VEGF of ControlshRNA; n=3–6).