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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Angiogenesis. 2020 Aug 11;23(4):685–698. doi: 10.1007/s10456-020-09740-y

Fig. 6. Direct binding of Rac1GTP to GRD of IQGAP sustains Rac1 activation.

Fig. 6.

Co-immunoprecipitation of GTP-Rac1 and IQGAP1, and GTP-Rac1 determined by immunoprecipitation with anti-GTP-Rac1 and western blot of total Rac1 in CECs transfected with plasmid DNAs expressing full length IQGAP1 (GFP-IQ-WT) or mutant GRD (GFP-IQ-MK24) and treated with VEGF for for 15, 30, 120 mins or PBS control (a, representative gel images, and b, quantification of densitometry; **p<0.01, ***p<0.001 vs. PBS of GFP-IQ-WT, †††p<0.001 vs. PBS of GFP-IQ-MK24; n=3).