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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Angiogenesis. 2020 Aug 11;23(4):685–698. doi: 10.1007/s10456-020-09740-y

Fig. 7. IQGAP1 regulates Rac1 activation by mediating Src activation.

Fig. 7.

(a and b) Western blots of phosphorylated Src (p-Src), total Src and β-actin in RPE/choroids of Iqgap1+/+ and Iqgap1−/− mice 7 days post laser treatment (*p<0.05 vs. Iqgap1+/+ mice; n=6–8; a, representative gel images and b, quantification of densitometry); (c-d) western blots of p-Src, total Src, IQGAP1 and β-actin and co-immunoprecipitation of VEGFR2 and Src, and western blots of total VEGFR2, p-Src total Src and β-actin in CECs transfected with IQGAP1 siRNA or control siRNA and treated with VEGF for 15 or 30 mins; and (e-f) activation of Rac1 determined by immunoprecipitation of anti-GTP-Rac1 and western blot of total Rac1, and western blots of total Rac1, p-Src, total Src and β-actin in CECs pretreated with Src inhibitor, Saracatinib (10 nM), for 30 mins followed by VEGF treatment for 30 or 60 mins (††p<0.01 vs. PBS of ControlsiRNA, ‡‡‡p<0.001 vs. PBS of IQGAP1siRNA, $ $p<0.01, $ $ $p<0.001 vs. PBS of Control, n=3; c and e, representative gel images, and d and f, quantification of densitometry).