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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Toxicology. 2020 Aug 13;443:152561. doi: 10.1016/j.tox.2020.152561

Fig 4. Eclosion and flight defects following RNAi-mediated knockdown of kon-tiki (kon).

Fig 4.

(A - B) Average percent eclosion ± SEM following (A) pan-muscle or (B) IFM-restricted knockdown of kon compared to genotype controls. Letters indicate pair-wise significant differences where p < 0.01, ANOVA, n = 150 pupae per treatment. (C - D) RT-PCR quantification of knockdowns showing average ± SEM of log-2-fold changes in gene expression. Letters indicate pair-wise significant differences, where p < 0.05, ANOVA, n = 3 pooled samples). (E - F) Flight function following IFM-restricted knockdown of kon in (E) adult male and (F) female mδ>kon-RNAi(JF) or mδ>w1118 flies. The horizontal line and error bars represent mean ± SEM of each sex. Asterisks indicate pair-wise significant differences where p < 0.01 (Mann-Whitney test, n ≥ 100 flies per group). (G – H) Representative images of IFM morphology from 49 - 57 h APF pupae following (H) IFM-restricted knockdown of kon (mδ:RFP>kon-RNAi) compared to (G) control (mδ:RFP>w1118).