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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Toxicology. 2020 Aug 13;443:152561. doi: 10.1016/j.tox.2020.152561

Fig. 6. Eclosion and flight defects following overexpression of kon.

Fig. 6

(A - D) Panmuscle kon overexpression. A) Eclosion ability is reduced by 72% in Mef2>Kon-HA compared to Mef2>w1118 controls (*p < 0.01, Z-test, n = 150 pupae per group. B) RT-q-PCR quantification of average ± SEM log-2-fold change in kon expression Mef2>Kon-HA pupae compared to Mef2>w1118 controls, significant difference indicated by asterisk (*) where p < 0.05, t-test, n = 3 independent samples. (C) The morphology of control Mef2:RFP>w1118 pupal IFM compared to D) Mef2:RFP>kon-HA at 49 – 57 h APF. White asterisks (*) indicate myospheres. (E - J) IFM-restricted overexpression of kon. E) Eclosion ability does not differ between control (mδ>w1118) and (mδ>kon-HA). F) RT-q-PCR quantification of the average ± SEM log-2-fold change in gene expression of kon in mδ>kon-HA pupae compared to mδ>w1118 controls, significant difference indicated by asterisk (*) where p < 0.05, T-test, n = 3 independent samples. G) The morphology of the pupal IFM with in control pupae and H) kon overexpression (mδ:RFP>kon-HA) is indistinguishable. I) Flight function in adult males and J) females is significantly reduced. Each point is the landing height of an individual fly, and the mean ± SEM is indicated by the horizontal black line and error bars. Asterisks indicate pair-wise significant differences where p < 0.01 (Mann-Whitney test, n ≥ 100 flies per group).