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. Author manuscript; available in PMC: 2021 Sep 28.
Published in final edited form as: Anal Methods. 2020 Sep 9;12(36):4404–4417. doi: 10.1039/d0ay01194k

Figure 8.

Figure 8.

(A) The sensitivity and the linear dynamic range of the nLC-MS method were benchmarked against a corresponding narrow-bore HPLC method in both positive- and negative-ionization mode for different lipid standards that were supplemented to a yeast lipid extract. The dynamic ranges of the respective classes were extrapolated using following standards: LCB_a from LCB 17:1;2 LCB_b from LCB 17:0;2, LCBP_a from LCBP 17:0;2; LCBP _b from LCBP 17:1;2; Cer and CerP from 18:1;2/12:0;0; LPS, LPG and LPA from their 17:1 species, LPC from LPC 13:0; PA, PC, PE, PG, PI and PS from their 17:0/14:1 species; CL from CL 15:0(3)/16:1; DAG from DAG 17:0/17:0 d5; TAG from TAG 17:0–17:1–17:0 d5. (B) and (C) Show the MS response (peak area) of the standards GlcCer 18:1;2/12:0;0 and PE 17:0/14:1 in dependency of the injection amount. Reprinted with permission from N. Danne-Rasche, C. Coman and R. Ahrends, Analytical Chemistry, 2018, 90, 8093-8101.