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. Author manuscript; available in PMC: 2020 Oct 2.
Published in final edited form as: Adv Healthc Mater. 2020 Aug 2;9(18):e2000754. doi: 10.1002/adhm.202000754

Figure 7.

Figure 7.

NPCs cultured in low degradability hydrogels maintain expression of neurotrophins and downregulate expression of senescence markers. A) NGF and CNTF expression are higher for NPCs cultured in low degradability gels compared to high degradability gels after 7 d in culture. B) mRNA expression for the senescence markers p16, p21, p53, and p19 decreases over 28 days of culture in low degradability gels. C) Quantitative RT-PCR analysis of NPCs cultured in low degradability hydrogels reveals sustained expression of NGF and GDNF, increased expression of NT-3 and NT-4/5, and decreased expression of BDNF and CNTF mRNA over 28 days in culture. D) Expression of neurotrophin mRNAs by NPCs cultured in high degradability gels follows similar trends as NPCs in low degradability gels. In (A), data are presented as mean ± s.d. n = 3 independent gels. *p < 0.05, ****p < 0.0001, two-tailed Student’s t-test. In (B)-(D), data are presented as geometric mean with 95% confidence intervals. n = 3–4 independent gels. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, compared to mRNA concentration at day 0, one-way ANOVA with Bonferroni post hoc test.