Figure 4 – NFAT induces ITPR3 expression after hepatic ischemia reperfusion.
(a) Immunohistochemistry for 5-methylcytosine (5mC; brown staining) in liver slices of control and ischemia-reperfusion animals at different time points. (b) DNA methylation levels (percentage) of ITPR3 promoter region. (c) Immunohistochemistry for NFAT (in brown) showing its activation. (d) Binding sites for NFAT in human 1 kb ITPR3 promoter region. (e) Luciferase assay shows that NFAT increases human ITPR3 promoter activation. Scale bars, 50 and 25 μm. Values expressed as mean ± SEM, n=5–7, *p<0.05 **p<0.01*** p<0.001.