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. 2020 Sep 18;7:549860. doi: 10.3389/fmed.2020.549860

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Copyright © 2020 Peng, Lu, Song, Vallance, Jacobson, Yu and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of different pretreatments for detection of SARS-CoV-2 from sputum. (A) Ct-values of the N gene from clinical sputum specimens under different treatments. (B) Ct-values of the ORF1ab gene from clinical sputum specimens under different treatments. Statistical differences were calculated using one-way ANOVA with post-hoc turkey multiple comparison test. For clinical sputum specimens showing no amplication curves, Ct-values of 50 were arbitrarily assigned to calculate statistical differences. **p < 0.01; ns, not significant. NALC, N-Acetyl-L-Cysteine; PK, Proteinase K; DTT, Dithiothreitol.