Figure 4.

Evaluation of cell viability and death under 48-h β-arrestin 2 overexpression. HEK293, hGL5, and A2780 cells were transfected by increasing amount of plasmid encoding β-arrestin 2 and cell viability and death were assessed by MTT and propidium iodide, respectively. Total protein content from 0.5 × 10⁴ 70% confluent log phase cells were loaded. (A) Representative image of β-arrestin 2 detected in cell lysates by Western blotting using a specific rabbit antibody using β-actin as a normalizer. (B) Box and Whiskers plot of MTT data, collected from transfected HEK293 cells overexpressing increasing levels of β-arrestin 2, representing cell viability. (C) hGL5 cell viability evaluated by MTT. (D) β-arrestin 2-dependent cell viability of the A2780 ovarian cancer cell line. (E) Data from the β-arrestin 2-overexpressing HEK293, representing cell death, in propidium iodide-stained samples. (F) β-arrestin 2-overexpressing hGL5 cell death. (G) β-arrestin 2-overexpressing A2780 ovarian cancer cell death. Brightness/contrast of Western blotting pictures were adjusted uniformly in all panels. Asterisks indicate significantly different data distribution than mock-transfected (untransfected) cells (Kruskal-Wallis test and Dunn's correction for multiple comparisons; *P < 0.05; **P < 0.001; ***P < 0.0001; n = 8).