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. 2020 Sep 18;7:533936. doi: 10.3389/fvets.2020.533936

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Copyright © 2020 Fazarinc, Vrecl, Poklukar, Škrlep, Batorek-Lukač, Brankovič, Tomažin and Čandek-Potokar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemical and succinate dehydrogenase (SDH) activity-based classification of myofiber types in Krškopolje pig longissimus dorsi (LD) muscle. (A) Serial transverse sections of the LD muscle from pigs raised in the conventional indoor husbandry system (group C; A–D) or in organic conditions (group O; panels E–H) stained with the monoclonal antibodies (A,E) NLC-MHCs, (B,F) SC 71, and (C,G) BF-F3, specific for myofiber types I, II, and IIb, respectively; (D,H) succinate dehydrogenase (SDH) activity demonstrating the oxidative profile of myofibers. Scale bar, 200 μm is valid for all panels.