Figure 2.

In Vitro Characterization of Chimeric Molecules Produced by the Armed Viruses

The supernatants harvested from virus-infected cells were passed through 0.22 μM filters before they were used for the experiments. (A) Western blot detection of transgene expression, with anti-HA tag IgG as the first antibody. (B and C) Flow cytometry analysis of the selective binding activity of affibody-PL (B) and EGF-PL (C) to tumor cells. Tumor cells were sequentially incubated with the following: (1) chimeric molecule-containing or control supernatant, (2) anti-HA antibody of mouse origin, and (3) FITC- or PE-conjugated goat anti-mouse IgG. The cells were then subject to flow cytometry analysis.