Table 1.
Plasmids and strains used in current study.
| Plasmids and strains | Description | References |
|---|---|---|
| Plasmid | ||
| pEC-Ptac | rep from native plasmid pGA1(GenBank: X90817.2) of C. glutamicum, Kanar, Ptac, lacI | This work |
| pk18mobsacB | Kanar, sacB, Plac | [13] |
| pCas9-mcherry | RFP:Cas9, Ampr | [37] |
| Plac-DivIVA (part) | Plac-DivIVA (part), Kanar | This work |
| Plac-FtsZ (part) | Plac-FtsZ (part), Kanar | This work |
| pX-AB | rep from pBL1, Cmr, Ptac-hasA-hasB-Ter | [22] |
| pEC-AB | pEC-Ptac derivate, Ptac-hasA-hasB-Ter | This work |
| pEC-DivIVA | pEC-Ptac derivate, Ptac-divIVA-Ter | This work |
| pEC-FtsZ | pEC-Ptac derivate, Ptac-ftsZ-Ter | This work |
| pEC-AB-DivIVA | pEC-Ptac derivate, Ptac-hasA-hasB-divIVA-Ter | This work |
| pEC-AB-FtsZ | pEC-Ptac derivate, Ptac-hasA-hasB-ftsZ-Ter | This work |
| pEC-RFP:AB | pEC-Ptac derivate, Ptac-rfp:hasA-hasB-Ter | This work |
| pEC-RFP:AB-FtsZ | pEC-Ptac derivate, Ptac-rfp:hasA-hasB-ftsZ-Ter | This work |
| Strain | ||
| E.coli TOP10 | F-mcrAΔ(mrr-hsdRMS-mcrBC) ϕ80 lacZ ΔM15 Δlac X74 recA1 deoR araD139 Δ(ara-leu) 7697 galU galK rpsl (strR) endA1 nupG |
Solarbio |
| C. glutamicum ATCC13032 | Wild type | [22] |
| C. glutamicum-PdivIVA: Plac | Wild type derivate, PdivIVA replaced by Plac, Kanar | This work |
| C. glutamicum-PftsZ: Plac | Wild type derivate, PftsZ replaced by Plac, Kanar | This work |
| C. glutamicum/pX-AB | Wild type derivate, pX-AB, Cmr | [22] |
| C. glutamicum-PdivIVA: Plac/pX-AB | C. glutamicum-PdivIVA: Plac derivate, pX-AB, Kanar, Cmr | This work |
| C. glutamicum-PftsZ: Plac/pX-AB | C. glutamicum-PftsZ: Plac derivate, pX-AB, Kanar, Cmr | This work |
| C. glutamicum/pEC-DivIVA | Wild type derivate, pEC-DivIVA, Kanar | This work |
| C. glutamicum/pEC-FtsZ | Wild type derivate, pEC-FtsZ, Kanar | This work |
| C. glutamicum/pEC-AB | Wild type derivate, pEC-AB, Kanar | This work |
| C. glutamicum/pEC-AB-DivIVA | Wild type derivate, pEC-AB-DivIVA, Kanar | This work |
| C. glutamicum/pEC-AB-FtsZ | Wild type derivate, pEC-AB-FtsZ, Kanar | This work |
| C. glutamicum/pEC-RFP:AB | Wild type derivate, pEC-RFP:AB, Kanar | This work |
| C. glutamicum/pEC-RFP:AB-FtsZ | Wild type derivate, pEC-RFP:AB-FtsZ, Kanar | This work |