Table 2.
The estimated single-cell membrane area of each recombinant strain.
| Stain | Cell Shape | Length/(μm) | Diameter 1/(μm) | Diameter 2/(μm) | Surface area/(μm2/cell) |
|---|---|---|---|---|---|
| wild-type | rod shape | 1.5 ± 0.2 | 0.7 ± 0.1 | – | 3.9 ± 0.7 |
| C. glutamicum-PdivIVA: Plac | sphere | – | 1.2 ± 0.2 | – | 4.6 ± 1.1 |
| C. glutamicum/pEC-DivIVA | light bulb | 2.7 ± 0.4 | 1.3 ± 0.2 | 0.9 ± 0.1 | 11.4 ± 2.8 |
| C. glutamicum-PftsZ: Plac | long-rod | 2.0 ± 0.3 | 0.7 ± 0.1 | – | 5.0 ± 1.7 |
| C. glutamicum/pEC-FtsZ | dumbbell | 7.2 ± 1.2 | 1.2 ± 0.1 | 0.7 ± 0.1 | 20.2 ± 3.6 |
Note: The length and diameter parameters of single cell were estimated by microscopic observation. Tens of cells for each strain were observed and the mean value of the length and diameter was calculated. Different area formulas for various shapes were described in Fig. S3.