Figure 8.

The effects of PA on intestinal permeability and tight junction protein expression in ileal tissues. In vivo intestinal permeability and tight junction protein expression in ileal tissues were assessed at 24 h after treatment of LPS. (A) The concentration of FD10S in the serum of rats. The higher level of FD10S in the serum indicates a higher intestinal permeability, resulting from a reduced barrier function of the intestine. (B–C) The protein expressions of ZO-1, occluding, and claudin-1 were determined by western blot analysis. (D) The protein expressions of ZO-1, occluding, and claudin-1 were determined by immunohistochemistry and observed under a microscope of 200 and 400 magnifications. The data represent the averages ± S.D. Significant differences between the treatment and control groups are indicated as *P < 0.05, **P < 0.01, ***P < 0.001. Significance was determined by one-way ANOVA.