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. 2020 Sep 18;11:527744. doi: 10.3389/fphar.2020.527744

Figure 7.

Figure 7

BMAL-1 overexpression reverses TMJ-OA in rats. Ad-NC plasmid and Ad-bmal1 plasmid were injected IA into the TMJs of mice once per week for 4, 6, and 8 weeks. (A) Compared with that in rats transfected with Ad-NC-plasmid, the P-ERK, MMP3/13 and ADAMTS5 upregulation was significantly delayed in bmal1 overexpression rats. (B) qRT-PCR results indicated decreased bmal1 mRNA levels in vivo after Ad-bmal1 plasmid transfection. (C) Western blotting assay images show that CRD-induced P-ERK upregulation in cartilage was markedly abrogated in bmal1 overexpression rats. (D) The CRD-induced MMP3 mRNA upregulation in cartilage was markedly abrogated in bmal1 overexpression rats. (E) The CRD-induced MMP13 mRNA upregulation in cartilage was markedly abrogated in bmal1 overexpression rats. (F) The CRD-induced ADAMTS5 mRNA upregulation in cartilage was markedly abrogated in bmal1 overexpression rats. (G) The CRD-induced COL2 mRNA downregulation in cartilage was markedly abrogated in bmal1 overexpression rats. All experiments were performed in triplicate, and the results are expressed as the mean ± SD. *P < 0.05; **P < 0.01, ***P < 0.005. NS, not significant.