Fig. 5.

Down‐regulation of LMTK3 represses bladder cancer cell proliferation and migration. (A, B) MTT assay was used to detect the viability of bladder cancer cells in the si‐LMTK3 and si‐control groups. (C) Cell colony formation assay detects alteration of cell survival for EJ and UMUC3 in the si‐LMTK3 and si‐control groups. Clone number in each well was counted and statistically analyzed in the cell colony formation assay. (D) Cell migration analysis of the si‐LMTK3 and si‐control groups in bladder cancer cells. (E) Statistical analysis of cell migration. (F) Western blot analysis of proteins involved in the EMT pathway. Data represent the mean ± SD of three separate experiments. Differences between two groups were compared using Student’s t‐test. *P < 0.05, **P < 0.001. Scale bar: 200 μm.