Fig. 6.

Effects of FSH at concentrations of 0, 9, 30 and 90 ng·µL⁻¹ on type II collagen and the PI3K/AKT/NF‐κB signaling pathway in ATDC5 chondrocytes by western blotting. (A) Representative images of western blotting. (B) The protein expression levels of p‐PI3K/PI3K, p‐AKT/AKT, p‐NF‐κB/NF‐κB and p‐IKBα/IKBα. Each expression level was normalized with GAPDH (n = 3). Data are expressed as the SEM. Multiple comparisons were presented using one‐way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001 and ****P < 0.0001.