Fig. 4.

ERS inhibitors alleviated the promotion of apoptosis and cytoplasmic Ca²⁺ levels of C2C12 myotubes. After differentiated for 4 days, C2C12 myotubes were treated with different single‐time drug interventions for 24 h. (A) Measurements of JC‐1 red and green fluorescence were performed as described in Fig. 1. (B) Cytoplasmic Ca²⁺ concentration detection on rest status; (C) the protein expression and quantification of different intervention. These data are presented as the (mean ± SD) for three independent experiments. **P < 0.01 vs Control; ***P < 0.001 vs Control; ****P < 0.001 vs Control; ^# P < 0.05, ^## P < 0.01 vs NNC‐55. Data were analyzed using one‐way ANOVA and Dunnett's test.