Fig. 5.

TH knockdown induced elevated ERS and apoptosis in C2C12 myotubes. (A) qRT‐PCR analysis of TH mRNA expression after TH silencing treatment for 48 h. (B, C) Representative ThT (green) fluorescence microscopic images and quantification, showing the ERS levels in myotubes. (D) Representative photographs of morphological changes of C2C12 myotubes; (E, F) MMP analysis using JC‐1 staining by flow cytometry and the relative fluorescence quantification of the JC‐1 red:JC‐1 green ratio as described in Fig. 1. These data are presented as the (mean ± SD) for three independent experiments. **P < 0.01, ***P < 0.001, ****P < 0.0001 vs Control. Data were analyzed using one‐way ANOVA and Dunnett's test.