Figure 2.

Cis-elements and trans-acting factors that regulate expression of AR and AR-Vs. (A) Diagram of a minigene splicing reporter to mimic AR and AR-V7 alternative splicing. The reporter contains AR exon and flanking ~400 bp nucleotide sequence from AR intron 3. Factors found to directly recognize and regulate splicing of the reporter are illustrated. (B) Schematic of the endogenous AR gene including the 5′UTR, 3′UTR and exon CE3. Factors found to directly recognize and regulate splicing of endogenous AR pre-mRNAs are illustrated. UTR = untranslated region, CE3 = cryptic exon 3, PAS = polyadenylation site, CMV = human cytomegalovirus promoter.